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KMID : 0360220130540040639
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Journal of the Korean Ophthalmological Society
2013 Volume.54 No. 4 p.639 ~ p.644
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Effect of Nitric Oxide on Adhesion and Migration of Trabecular Meshwork Cells
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Bae Jin-Seong
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effect of nitric oxide (NO) on the adhesion and migration of cultured human trabecular meshwork cells (HTMC). Methods: For adhesion assay, primarily cultured HTMC were attached to culture dishes for 1 hr, cells were rinsed, and the remaining adherent cells were assessed with MTT assay. Degree of cellular migration was assessed under normal and stressed conditions using microchemoattraction chambers. Effect of NO on the adhesion and migration was assessed with or without co-exposure of S-nitroso-N-acetylpenicillamine (SNAP). Results: NO did not affect the degree of adhesion or migration of HTMC (p ＞ 0.05). The degree of adhesion increased although the degree of migration decreased with 1% serum (p < 0.05). Degrees of migrations decreased after mechanical stress (p < 0.05). Conclusions: NO may not affect the adhesion or migration of HTMC.
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KEYWORD
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Adhesion, Migration, Nitric oxide, Trabecular meshwork cells
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